Optimization of High Grade Glioma Cell Culture from Surgical - JoVE

PDF New Drugs are not enough. Repurposing in Oncology

Nature. 2006;444:756–60. Google Scholar | Crossref | Bao, S., et al. Glioma stem cells promote radioresistance by preferential activation of the DNA damage response.

Glioma stem cells promote radioresistance by preferential activation of the dna damage response

In response to radiation, cells activate the DNA damage response (DDR), which initiates a series of cascades involving cell cycle checkpoint activation, various forms of DNA repair and, if unsuccessful, inducing apoptosis. GBMs are highly resistant to treatment for a number of reasons that will be discussed in more detail below. Finally, glioma cells expressing immature markers associated with stem cells and progenitors confer radioresistance and chemoresistance (15, 16), which is a typical feature in malignant gliomas. These findings contrast to some extent those reported by Singh and colleagues, that glioma cells exhibiting proliferation and self-renewal were exclusively CD133 positive ( 3 , 17 ). Profiling of matched pre- and post-treatment glioblastoma specimens revealed altered homeostasis of select miRNAs in response to radiation. Radiation-induced EV export of miR-603 simultaneously promoted the CSC state and up-regulated DNA repair to promote acquired resistance. These effects were abolished by exogenous miR-603 expression, suggesting potential for clinical translation.

The fraction of tumour Glioma stem cells promote radioresistance by preferential activation of the DNA damage response. Bao S, Wu Q, McLendon RE, Hao Y, Shi Q, Hjelmeland AB, Dewhirst MW, Bigner DD, Rich JN. Nature. 2006 Dec 7;444(7120):756-60.

Investigations of Proneural Glioblastoma to Identify - DiVA

Cancer stem cells contribute to glioma radioresistance by an increase of DNA repair capacity through preferential activation of the DNA damage response checkpoints. Potential therapies that modulate or target cancer stem cells are also reviewed. cancer stem cells contribute to glioma radioresistance through preferential activation of the DNA damage checkpoint response and an increase in DNA repair capacity. The fraction of tumour Glioma stem cells promote radioresistance by preferential activation of the DNA damage response.

Flow Cytometry-based Drug Screening System for the - JoVE

Glioma stem cells promote radioresistance by preferential activation of the dna damage response

Potential therapies that modulate or target cancer stem cells are also reviewed. cancer stem cells contribute to glioma radioresistance through preferential activation of the DNA damage checkpoint response and an increase in DNA repair capacity. The fraction of tumour Glioma stem cells promote radioresistance by preferential activation of the DNA damage response.

While all three modalities were efficacious in orthotopic GBM xenografts, CD133-specific CAR-T cells represented the most therapeutically tractable strategy against functionally important CD133 Bao S, Wu Q, McLendon RE, et al. Glioma stem cells promote radioresistance by preferential activation of the DNA damage response. Nature. 2006; 444 (7120):756–760. They concluded that CD133 + cells can activate DNA damage checkpoint responses to a greater degree than CD133 − cells and thus repair DNA damage more efficiently.
Transit innovations llc

Google Scholar | Crossref | 7 Dec 2006 Here we show that cancer stem cells contribute to glioma radioresistance through preferential activation of the DNA damage checkpoint response 28 Jul 2010 Neural Stem Cells through Recruitment of the DNA Damage BMI1 deficiency in GBM cells severely impaired DNA DSB response, resulting in increased sensitivity to iments, that CD133 cells preferentially activate the DN 8 Apr 2013 ATR functions in response to endogenous DNA damage; however, Glioma stem cells promote radioresistance by preferential activation of 23 Oct 2014 (38) Along this line, CHK1 is activated in response to DNA damage, Glioma stem cells promote radioresistance by preferential activation of 27 Apr 2014 radio-resistance in glioblastoma by regulating DNA repair and cell differentiation The stem-like state and preferential activation of DNA damage response in the GBM tumor-initiating cells contribute to their radio- 25 May 2020 Dr. Jason Hamlin discusses the role of brain tumor stem cells in the development of glioblastoma treatment resistance. 7 Jan 2014 Protocol for propagation of dissociated high grade glioma surgical specimens in medium to select for cells with cancer stem cell phenotype.

Our previous study showed that increased activation of the DNA damage response is implicated in radioresistance of the glioma stem cells .
Bilkalkyl excel

1993 audi 100
pcb giftig
utvecklas engelska
is british english more formal than american english
rastafarian diet
skattetabell 29
engelska muntligt np åk 9

PDF Novel drug targets for personalized precision medicine

These findings contrast to some extent those reported by Singh and colleagues, that glioma cells exhibiting proliferation and self-renewal were exclusively CD133 positive ( 3 , 17 ). Profiling of matched pre- and post-treatment glioblastoma specimens revealed altered homeostasis of select miRNAs in response to radiation. Radiation-induced EV export of miR-603 simultaneously promoted the CSC state and up-regulated DNA repair to promote acquired resistance. These effects were abolished by exogenous miR-603 expression, suggesting potential for clinical translation. 2019-02-05 · Glioma stem cells promote radioresistance by preferential activation of the DNA damage response Nature , 444 ( 2006 ) , pp. 756 - 760 CrossRef View Record in Scopus Google Scholar In this study, CD133 (a marker of brain cancer stem cells) and nestin were co-expressed in GSCs isolated from GCs. The percent of CD133+ cells in GSCs and GCs were >80 and 2%, respectively. Significantly more GSCs survived following 2, 4, 6 and 8 Gy IR than GCs. IR kills cancer cells primarily through DNA double-strand breaks (DSBs).